CARBOHYDRATES METABOLISM IN THE BLOOD OF RATS WITH IMPAIRED GLUCOSE TOLERANCE UNDER LONG TERM MELATONIN INJECTIONS.

OBJECTIVE: The aim: To determine the influence of melatonin on the glucose level and content of malondialdehyde, activities of pyruvate kinase and glucose-6-phosphate dehydrogenase enzymes in the blood; histochemical features of glycogen distribution in liver of rats with impaired glucose tolerance. PATIENTS AND METHODS: Materials and methods: Diabetes in rats was induced by intra-abdominal injection of a 5% solution of alloxan monohydrate at the rate of 170 mg/kg of body weight. Four days after animals were divided into rats with impaired glucose tolerance and melatonin-group with impaired glucose tolerance (5 mg/ kg «Sigma¼ USA, daily and intraperitoneal for 42 days starting from 5th day). Impaired glucose tolerance was determined by measurement of glucose profiles - fasting <5.6 mmol/l; postprandial (2h post-load) 7.8 - 11.0 mmol/l. Histochemical examination of the liver was performed according to the standard method of PAS-reaction staining. Statistical analysis was performed using Statistica 10 StatSoft Inc. RESULTS: Results: Pyruvate kinase activity in erythrocytes and optical density of glycogen in hepatocytes of animals with impaired glucose tolerance decreased on 18% and 11%, activity of glucose-6-phosphate dehydrogenase and content of malondialdehyde increased on 35% and 23%, respectively compared with the control. We have reached the recovery of the pyruvate kinase and normalization of glucose-6-phosphate dehydrogenase activities, malondialdehyde levels, glucose profiles in the blood as well as glycogen distribution in the liver caused by melatonin injections. CONCLUSION: Conclusions: We have determined that long term melatonin injections did better glucose tolerance in rats.

FEATURES OF PRENATAL MORPHOGENESIS AND PECULARITIES OF THE UTRICULUS PROSTATICUS FETAL ANATOMY.

OBJECTIVE: Aim: To determine the peculiarities of the structural organization of the utriculus prostaticus (UP) in pre-fetuses and fetuses. PATIENTS AND METHODS: Materials and methods: The study of macroscopic features and microscopic peculiarities of the prostate gland and the prostatic part of the urethra was carried out on 46 sections of human pre-fetuses and fetuses aged from 9 weeks to birth (31,0-375,0 mm PCL). The work uses the method of microscopic study of serial histological and topographic-anatomical specimens of the prostate gland, as well as the method of the thin preparation of the prostate part of the urethra in fetuses of different ages and morphometry. RESULTS: Results: In 58,0-66,0 mm PCL fetuses the paramesonephric ducts are reduced, except of their connected caudal part, which is a morphological substrate for the development of the UP. At 72,0-79,0 mm PCL fetuses, cavity is replaced by cellular mass. At the 85,0-120,0 mm PCL fetuses, the UP connects with the lumen of the urethra. The cavity of the UP intensivelly proliferates with cells. In fetuses of 125,0-135,0 mm PCL is presente dense arrangement of glandular elements, which are surrounded by fibrous-muscular membrane. In fetuses of 150,0-160,0 mm PCL, in the caudal direction, the cavity of the UP gradually narrows, it forms invaginations, especially in the middle and lower parts, or is divided into separate, interconnected chambers. In fetuses of 170,0-185,0 mm PCL, UP has elongated-oval or rounded-oval shape. In the caudal direction, the UP is directed ventral to the colliculus seminalis and is located slightly anterior and superior to the ejaculatory ducts. In 8-month-old fetuses, the lumen of the UP is lined with a pseudostratified cubical epithelium, outside of which there is a tunica muscularis. Ejaculatory ducts lined with a two-layer cuboidal epithelium are placed on both sides of the UP. A 270,0 mm PCL fetus has no UP at the apex of the colliculus seminalis. In fetuses 315,0-335,0 mm PCL, the process of cavity formation spreads to new areas of glandular formations of the prostate gland and their final branches. Most of the glandular formations open into the prostatic part of the urethra directly below the UP and the distal parts of the ejaculatory ducts. Microscopic examination of frontal sections of the prostate gland of a fetus with a 360,0 mm PCL revealed a septum in the UP which divides the cavity of the UP into the right and left halves of a round-oval shape. CONCLUSION: Conclusions: The formation of utriculus prostaticus occurs from the paramesonephric ducts in the 11th week of fetal development. At the beginning of the 4th month of intrauterine development, it gradually decreases in size. From the middle of the 5th month of prenatal development, the utriculus prostaticus lengthens, and starting from the fetus of 7 months, both its length and width increase. At the end of the fetal period, the utriculus prostaticus acquires a round-oval shape, its length increases from 0,5 to 4,3 mm during prenatal ontogeny.

VARIATIONS OF THE STRUCTURE, TOPOGRAPHY, BLOOD SUPPLY AND INNERVATION OF THE BRACHIORADIALIS IN HUMAN FETUS.

OBJECTIVE: The aim of the research was to establish the features of age-related and individual anatomical variability of the brachioradialis, its blood supply, and innervation options. PATIENTS AND METHODS: Materials and methods: The study of the variant anatomy of the brachioradialis and its vascular and nervous structures was carried out on 25 preparations of human fetuses of 4-7 months, 81.0-270.0 mm parietal-coccygeal length (PCL) using macromicroscopic preparation, injection vessels, and morphometry. RESULTS: Results: Spindle-like (56%) and round (24%) shapes of the brachioradialis were found in most of the studied fetuses; its elongated flat (12%) and triangular (8%) forms occur less often. In a fetus of 185.0 mm TKD, the right brachioradialis consisted of two separate parts: upper and lower, which were connected at the level of the middle of the forearm into a common short muscle belly. In another human fetus of 220.0 mm TKD, the right brachioradialis also consisted of two separate parts - upper and lower, triangular in shape, but separated by a pronounced horizontal gap. CONCLUSION: Conclusions: The features of the fetal anatomy of the brachioradialis are established: its variability and bilateral asymmetry of shape and size, variability of the places of origin and attachment, etc. In individual human fetuses, the brachioradialis consists of two separate parts that have special topographical relationships. The area of the greatest concentration of both extra- and intramuscular nerves and arteries is the upper and middle third of the brachioradialis. In the area of the forearm, the course of radial vascular-nerve formations is determined by the brachioradialis.

ONTOGENETIC ASPECTS OF MELATONIN RECEPTORS 1A DENSITY IN THE LATERAL PREOPTIC NUCLEUS OF THE HYPOTHALAMUS UNDER VARIOUS LIGHT CONDITIONS.

OBJECTIVE: The aim: To find out density of melatonin receptors 1A in the neurons of the lateral preoptic nucleus of the hypothalamus in mature and old rats under various light conditions. PATIENTS AND METHODS: Materials and methods: The study was carried out on 72 albino mature and old rats with light conditions appropriate for the experiment. To find out circadian differences of melatonin receptors 1A the material for the study was taken at 2 p.m. and 2 a.m. Visualization of primary antibodies against melatonin receptors 1A (Abcam) was conducted by means of the polymeric system Dako and diaminobenzidine staining under the microscope Delta Optical Evolution 100. The intensity of staining was assessed on the digital copies of images according to computer microdensitometry method. RESULTS: Results: Immunohistochemical examinations conducted enable to suggest that melatonin receptors 1A in the neurons of the lateral preoptic nucleus of the hypothalamus respond to different light conditions. In particular, intensity of immunohistochemical staining to melatonin receptors 1A under conditions of light deprivation increases both in mature and old rats, but it decreases under conditions of light stimulation. The parameter is higher at 2 a.m. as compared with 2 p.m. Intensity of immunohistochemical staining to melatonin receptors 1A in the neurons of the lateral preoptic nucleus of the hypothalamus is always lower in old rats than in mature ones. CONCLUSION: Conclusions: Density of melatonin receptors 1A in the neurons of the lateral preoptic nucleus of the hypothalamus is subordinated to the circadian rhythm: it increases at night and decreases in the daytime. At the same time, light stimulation results in disorders of the rhythm and development of desynchronization.

BIOCHEMICAL AND MORPHOLOGICAL MARKERS OF EXPERIMENTAL SCOPOLAMINE-INDUCED NEURODEGENERATION AND THE EFFECT OF ENALAPRIL ON THEM.

OBJECTIVE: The aim: Was to study enalapril effect on protein peroxide oxidation and lipids of the cerebral cortex and hippocampus of rats with neurodegeneration pattern. PATIENTS AND METHODS: Materials and methods: The experiments were conducted on nonlinear albino male rats 0,18-0,20 kg of the body weight. Modeled neurodegeneration was simulated by intraperitoneal introduction of scopolamine hydrochloride during 27 days in the dose of 1 mg/kg. Enalapril was introduced intraperitoneal in the dose of 1 mg/kg. RESULTS: Results: In rats with modeled scopolamine-induced neurodegeneration in the cerebral cortex and hippocampus under enalapril effect, the content of products, reacting with 2-thiobarbituric acid and proteins of a neutral and major character, decreases, which is indicative of a reduced intensity of lipid and protein peroxide oxidation. Morphologic the number of cells with karyopyknosis signs decreases and a relative staining density of the neuron tigroid substance increases, which is indicative of inhibition of the progress of the cerebral neurodestructive processes under conditions of scopolamine-induced damage. CONCLUSION: Conclusions: The obtained results are indicative of enalapril ability to improve the examined indices, which is the evidence of increased processes of antioxidant protection and possibility to inhibit neurodegeneration development in case of scopolamine induced lesion of the brain.